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(사)한국바이오칩학회 The Korean BioChip Society

BT+IT+NT융합시대의 리더 : 한국바이오칩학회

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Info. Vol.1 - No.3 (2007.09.20)
Title Micropatterning of Lipid Bilayers in a Microfluidic System via Forced Vesicle Fusion
Authors Yu-Jung Kim1, Sankar Thirunavukkarasu1, Joo Young Park1 & Hyungil Jung1
Institutions 1Department of Biotechnology, Yonsei University, Seoul 120-749, Korea Correspondence and requests for materials should be addressed to H. Jung (hijung@yonsei.ac.kr)
Abstract Substrate-supported planar lipid bilayers (SPB) are excellent model for bio-membranes, and have potential scientific and technological applications. Due to these features, SPBs have been studied to determine if their application can be useful for biological analyses, such as the evaluation of cell to cell interactions or as cell based biosensors. In this study, the patterning of 1, 2-bis(10, 12-tricosadiyno-yl)-snglycero-3-phosphocholine (diynePC) bilayer that is a polymerizable lipid was evaluated via forced vesicle fusion in a microfluidic system. The formation of diynePC lipid bilayers in the microfluidic channel was sensitive to flow rate, and homogeneous diynePC lipid bilayers were formed when diynePC was applied at a flow rate of 0.1 關L/min for 10 min. These layers were patterned via selective photopolymerization using a UV dose of 4.1 J/cm2. Because lipids in areas blocked from UV can be selectively removed by ethanol, new lipid layers, such as egg yolk phosphatidylcholine (egg PC), that retain some characteristic features of their native cellular membrane, can be incorporated into the areas that were cleared by ethanol. This micropatterning of diynePC lipid bilayers within the microfluidic system can be used to provide a platform for a lipid-based immunoassay chip, and may therefore be a useful tool for cell based high-throughput systems.
Keyword Forced vesicle fusion, Infusion rate, Photopolymerization, Microfluidic system, Micropatterning
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