| Info. | Vol.7 - No.2 (2013.06.20) |
|---|---|
| Title | Analysis of Plk1-Bora interaction using a Protein Chip system |
| Authors | Jung-kyu Lee1 & In-Cheol Kang1,2 |
| Institutions | 1Department of Biological Science and BioChip Research Center,
Hoseo University, Asan 336-795, Korea 2Innopharmascreen Inc., Asan 336-795, Korea |
| Abstract | We investigated the interaction between Polo-Box Domain (PBD) of polo-like kinase 1 (Plk1) and Bora peptide based on protein-protein interactions using the Proteochip (Proteogen, Korea) technology. Plk-1 protein conjugated with GST was bound to anti-GST antibody immobilized on the surface of the Proteochip, after which a Bora peptide labeled with a fluorescent dye, Cy5, was interacted with the Plk-1 protein on the chip in a dose-dependent manner. A chemical compound E8 was screened as a novel inhibitor against the Plk1-Bora interaction using the Proteochip. The compound significantly inhibited NCI-H460 (lung cancer) cell proliferation in vitro in a dose-dependent manner. The protein chipbased Plk1-Bora binding system will serve a novel promising tool which is applicable for screening of novel inhibitors against Plk1, regardless of the enzyme activity. |
| Keyword | Plk1, ProteochipTM, Bora peptide, Proteinprotein interaction, Thymoquinone |
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