| Info. | Vol.7 - No.4 (2013.12.20) |
|---|---|
| Title | Optical immunosensor for quantifying C-telopeptide fragments of type II collagen as an osteoarthritis biomarker in urine |
| Authors | Su Jin Kim1,??/sup>, Yoo Min Park1,??/sup>, Byoung-Hyun Min1,2, Dae-Sik Lee3 & Hyun C. Yoon1 |
| Institutions | 1Department of Molecular Science & Technology, Ajou University, Suwon 443-749, Korea 2Department of Orthopedic Surgery, School of Medicine, Ajou University, Korea 3IT Convergence Components Laboratory, ETRI, Daejeon 305-350, Korea |
| Abstract | A fluorescence-based biosensor for measuring the quantity of C-telopeptide fragments of type II collagen (CTX-II) was developed as an osteoarthritis biomarker in urine. During osteoarthritis progression, joint components such as cartilage collagen are degraded by collagenase-protease and secreted into the serum as CTX-II. To detect CTX-II in urine (uCTXII) having structural heterogeneity, conventional sandwich format assay does not provide sufficient accuracy. As an alternative, a competitive immunoassay was developed for the quantification of uCTX-II, which uses antibody-conjugated fluoro-microbeads to generate an optical signal. After preparing the biosensing surface, the uCTX-II sample and antibody-optical probe conjugates were reacted on the PEG4-EKGPDP exposed biosensing surface. The optical probes competed with the uCTX-II in the sample for binding to the immobilized PEG4-EKGPDP. This assay was able to detect uCTX-II concentrations between 200 ng/mmol (corrected value vs. creatinine) and 1,400 ng/mmol, encompassing the clinical detection ranges required for osteoarthritis diagnosis. The competitive immunoassay developed for uCTX-II detection was rapid and exhibited good correlation with conventional ELISA methods. This novel competition assay is a promising tool for the diagnosis and monitoring of osteoarthritis using urine samples. |
| Keyword | Fluorescent microbeads, Competition immunoassay, uCTX-II, Osteoarthritis |
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