Go to contents

(사)한국바이오칩학회 The Korean BioChip Society

BT+IT+NT융합시대의 리더 : 한국바이오칩학회

학술지 열람

논문 정보 자세히 보기

Info. Vol.8 - No.2 (2014.06.20)
Title Isolating and Concentrating Rare Cancerous Cells in Large Sample Volumes of Blood by Using Dielectrophoresis and Stepping Electric Fields
Authors Guang-Hong Chen1, Ching-Te Huang1, Hsin-Hui Wu1, Tatyana N. Zamay2,3, Anna S. Zamay2,3 & Chun-Ping Jen1,*
Institutions 1Department of Mechanical Engineering and Advanced Institute of Manufacturing with High-tech Innovations, National Chung Cheng University, Chia Yi, Taiwan, R.O.C.
2Department of Biological Chemistry, Krasnoyarsk State Medical University, Krasnoyarsk, Russia
3Small innovative enterprise ?쏮edBioTeh?? Krasnoyarsk, Russia
Abstract Detecting rare cells, such as circulating tumor cells (CTCs), circulating fetal cells, and stem cells, is vital during medical diagnostics and characterization. During carcinogenesis, cancer cells detach from the primary tumor into the blood stream, becoming CTCs. Typical rare cell samples are considered any sample that contains less than 1000 target cells per milliliter. The volumes of microfluidic devices typically range from several microliters to nanoliters; this is excessively small for experimenting using lowconcentration samples. This study involved isolating cancerous cells in an open-top chamber with sub-milliliter volumes (0.1 mL) of blood samples by using a lysis buffer solution for red blood cells (RBCs), as well as concentrating cells employing the dielectrophoretic force generated using stepping electric fields, which were produced using a handheld electric module that comprised a voltage-frequency converter and an operational amplifier. To increase the sample volume, an open-top chamber was fabricated on and bonded to a glass substrate by using circular microelectrodes. The concentrations of cancer cells and RBCs were adjusted to 500 cells/mL and 4횞105 cell/mL, respectively, for the experiments. To reduce the interference of blood cells during detection and isolate CTCs, the RBCs in the sample were lysed in a lysis buffer solution before the proposed chip was used to dielectrophoretically manipulate the rare cancerous cells. The findings indicated that the lysis buffer lysed the erythrocytes and the survivability levels of the cancerous cells (HeLa and MCF-7) remained high in the lysis buffer. The positive dielectrophoretic cancerous cells were guided based on the direction of the stepping electric field because of movement in the high-electric-field region; hence, the cancerous cells concentrated and collected at the central electrode.
Keyword Dielectrophoresis (DEP), Circulating tumor cells (CTCs), Concentration, Isolation, Stepping electric field
PDF File
# 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다.
→ 전체 목록 보기

(사)한국바이오칩학회 (The Korean BioChip Society)
전화 : 070-7767-9855
전자우편 : biochip@biochip.or.kr
전화 : 070-7767-9867
전자우편 : biochip2@biochip.or.kr
주소 : (우)06130, 서울특별시 강남구 테헤란로7길 22 과학기술회관 신관 804호     대표자 : 김민곤, 고유번호 : 206-82-65403
팩스 : 02-921-9856 , 웹사이트 : http://www.biochips.or.kr Copyright © The Korean BioChip Society. All Rights Reserved.