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Info. Vol.8 - No.2 (2014.06.20)
Title A Highly UV-transparent Fused Silica Biochip for Sensitive Hepatotoxicity Testing by Autofluorescence
Authors Michael Fritzsche1, Joachim Fritzsche2, Jonas O. Tegenfeldt3 & Carl-Fredrik Mandenius1,*
Institutions 1Division of Biotechnology/IFM, Link?ing University, 581 83 Link?ing, Sweden
2Department of Physics, University of Gothenburg, 41296 Gothenburg, Sweden
3Division of Solid State Physics, Lund University, 22100 Lund, Sweden
Abstract Fabrication and application of a non-fluorescent and UVtransparent microfluidic biochip in fused silica that allows sensitive autofluorescence detection are described. The biochip is particularly useful in cell-based assays where the most informative autofluorescence signals from the cells reside in the ultraviolet spectral range and where plastic labware materials commonly used in cell culture work severely disturb such measurements. In this study the fused silica biochip was used for measuring intrinsic autofluorescence from liver cells in order to assess hepatotoxic effects of drugs. The assessment assay was carried out with the human liver cell line HepG2 under perfusion conditions in the microfluidics of the biochip. The autofluorescence from the liver cells exposed to quinidine was readily recorded without background disturbance and correlated well with reference toxicity
data.
Keyword Autofluorescence, Drug testing, Microfluidic chip, Fused silica, In vitro toxicity, HepG2 cell line
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