| Info. | Vol.8 - No.3 (2014.09.20) |
|---|---|
| Title | A Protein Chip Based Inhibitor Screening for Influenza Neuraminidases: the Importance of Glycan-specific Recognition |
| Authors | Jung-kyu Lee1, Chan-Won Park2, Hyuk-Ku Kwon3, Seunho Jung4, Hyun-Ja Jeong5, In-Cheol Kang1 & Youngjin Choi5,* |
| Institutions | 1Department of Biological Science & BioChip Research Center, Hoseo University, Asan 336-795, Korea 2Innopharmascreen Inc., Asan 336-795, Korea 3Department of NanoBiotronics, Graduate School of Hoseo University, Asan 336-795, Korea 4Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center & Center for Biotechnology Research in UBITA, Konkuk University, Seoul 143-701, Korea 5Department of Food Science and Technology & BioChip Research Center, Hoseo University, Asan 336-795, Korea |
| Abstract | A protein chip assay system based on a specific carbohydrate protein interaction has been constructed for the identification of chemical inhibitors of influenza neuraminidase. The devised chip is composed of three layered proteins, that is, a matrix, a probe, and a neuraminidase protein. Transferrin, the matrix protein, was immobilized onto a chip panel to provide biological glycan chains. Fluorescence-labeled lectin or hemagglutinin was added as a glycan-interacting protein probe. Finally specific sialic acid moiety on the transferrin was then cleaved by an avian or human influenza neuraminidase. Each lectin or hemagglutinin showed different glycan specificity as a result of catalytic action of different influenza neuraminidase. Using this principle, we finally tested the neuraminidase of strain H1N1 as a model system to verify the screening capability of this protein chip approach. |
| Keyword | Carbohydrate-protein interaction, Drug screening, Neuraminidase, Protein chip, Anti-viral |
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