| Info. | Vol.8 - No.3 (2014.09.20) |
|---|---|
| Title | Protein Chip-based GLP-1 Receptor Assay System |
| Authors | Chanwon Park1 & In-Cheol Kang1,2,* |
| Institutions | 1Department of Biological Science and BioChip Research Center, Hoseo University, Asan 336-795, Korea 2Innopharmascreen Inc., Asan 336-795, Korea |
| Abstract | We investigated GLP-1 Receptor (GLP-1R) assay system on the basis of receptor-ligand interaction using ProteoChip. GLP-1R was immobilized on the surface of the ProteoChip and then GLP-1 peptide labeled with a Cy5-fluorescent dye was interacted with the GLP-1R array on the chip in a dose-dependent manner. Exendin-4 was tested for targeting GLP-1R to demonstrate the chip-based GLP-1R assay system. Chemical compound 6 and 45 were screened as novel agonists of GLP-1R using the GLP-1R assay system. The compound significantly inhibited GLP-1 binding to GLP-1R in a dose-dependent manner. The protein chip-based GLP-1R assay system will serve as a useful tool for screening of novel GLP-1R agonists. |
| Keyword | GLP-1R, GLP-1, Exendin-4, ProteoChipTM, Protein-protein interaction |
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