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Info. Vol.9 - No.2 (2015.06.20)
Title Measurement of Prostate-Specific Antigen Level as a Biomarker for Breast Cancer by Using Mass Signal Amplification
Authors Jungchan Nam1, Minyoung Yoo1 & Woon-Seok Yeo1,*
Institutions 1Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea
Abstract Recently, it has been known that prostatespecific antigen (PSA) can be a useful biomarker for the diagnosis with breast cancer as well as that with prostate cancer. The level of PSA in patients of breast cancer is much lower than that in patients of prostate cancer, and therefore, the quantitative method of PSA with ultra-high sensitivity is required for the breast cancer diagnosis. In this paper, we introduce an efficient method for selective detection and quantification of PSA using gold nanoparticles (AuNPs), biochips, and mass spectrometry (MS). The PSA in a sample was captured by AuNPs and a biochip which present antibodies through a sandwich assay format. The AuNPs carried a huge number of reporter molecules, called amplification tag (Am-tag), and the presence of PSA in the sample was then verified through amplified mass signal of Am-tag by laser desorption/ionization timeof-flight (LDI-TOF) MS analysis to enable ultrasensitive detection of PSA. In addition, PSA in the sample was quantitated by using an internal standard (IS) which was prepared with AuNPs and deuterium-labeled Amta (dAm-tag) which has a heavier molecular weight. Therefore, comparing the mass intensities between the Am-tag and the dAm-tag allowed quantification of PSA. This method was validated through the quantification of PSA in the female human serums with satisfactory results. We believe our method to detect and analyze PSA quantitatively and highly sensitively will be applied to patients??serum for the diagnosis of breast cancer.
Keyword Prostate-specific antigen, Gold nanoparticle, LDI-TOF MS, Self-assembled monolayers
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