Go to contents

(사)한국바이오칩학회 The Korean BioChip Society

BT+IT+NT융합시대의 리더 : 한국바이오칩학회

학술지 열람

논문 정보 자세히 보기

Info. Vol.9 - No.2 (2015.06.20)
Title Measurement of Prostate-Specific Antigen Level as a Biomarker for Breast Cancer by Using Mass Signal Amplification
Authors Jungchan Nam1, Minyoung Yoo1 & Woon-Seok Yeo1,*
Institutions 1Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea
Abstract Recently, it has been known that prostatespecific antigen (PSA) can be a useful biomarker for the diagnosis with breast cancer as well as that with prostate cancer. The level of PSA in patients of breast cancer is much lower than that in patients of prostate cancer, and therefore, the quantitative method of PSA with ultra-high sensitivity is required for the breast cancer diagnosis. In this paper, we introduce an efficient method for selective detection and quantification of PSA using gold nanoparticles (AuNPs), biochips, and mass spectrometry (MS). The PSA in a sample was captured by AuNPs and a biochip which present antibodies through a sandwich assay format. The AuNPs carried a huge number of reporter molecules, called amplification tag (Am-tag), and the presence of PSA in the sample was then verified through amplified mass signal of Am-tag by laser desorption/ionization timeof-flight (LDI-TOF) MS analysis to enable ultrasensitive detection of PSA. In addition, PSA in the sample was quantitated by using an internal standard (IS) which was prepared with AuNPs and deuterium-labeled Amta (dAm-tag) which has a heavier molecular weight. Therefore, comparing the mass intensities between the Am-tag and the dAm-tag allowed quantification of PSA. This method was validated through the quantification of PSA in the female human serums with satisfactory results. We believe our method to detect and analyze PSA quantitatively and highly sensitively will be applied to patients??serum for the diagnosis of breast cancer.
Keyword Prostate-specific antigen, Gold nanoparticle, LDI-TOF MS, Self-assembled monolayers
PDF File
# 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다.
→ 전체 목록 보기

(사)한국바이오칩학회 (The Korean BioChip Society)
주소 : (우)06130, 서울특별시 강남구 테헤란로7길 22 과학기술회관 신관 804호     대표자 : 심상준, 고유번호 : 206-82-65403
대표전화 : 070-7767-9855 , 팩스 : 02-921-9856 , 전자우편 : biochip@biochip.or.kr, 웹사이트 : http://www.biochips.or.kr Copyright © The Korean BioChip Society. All Rights Reserved.
Facebook Twitter URL 복사