| Info. | Vol.1 - No.4 (2007.12.20) |
|---|---|
| Title | Identification of Lamivudine-resistant Hepatitis B Virus by Oligonucleotide Microarray |
| Authors | Jung-A Kang1, Jae-Chan Park1,3, Jong-Man Kim1, Young-Guy Chai3 & Kyoung-Ryul Lee1,2 |
| Institutions | 1BioCore Institute of Biotechnology BioCore Co., Seoul 2Seoul Clinical Laboratories (SCL) & Seoul Medical Science Institute, Seoul 3Department of Nanobiotechnology, Hanyang Univ, Ansan, Korea Correspondence and requests for materials should be addressed to K.-R. Lee (dnachip@bio-core.com) |
| Abstract | Hepatitis B virus (HBV) is a partially double-stranded DNA virus that infects the human liver, causing cirrhosis and hepatocellular carcinoma. The nucleoside anal-ogue lamivudine is an effective antiviral agent for HBV; however, drug-resistant HBV strains may emerge during prolonged lamivudine treatment. Lamivudine- resist-ant strains exhibit specific mutations, especially in the B and C domains of the HBV polymerase gene. The ability to detect such mutations is of increasing importance with increased availability of alternative antiviral agents such as penciclovir or famiciclovir, which show promise as a treatment for chronic HBV infections. We have developed a rapid and accurate HBV drug-resistant oligonucleotide chip for the detection of specific mutations in the HBV polymerase gene. Synthesized oligonucleotide probes of 25-32 bases in length with modifications at the 5?셞nd were spotted in duplicate and covalently linked onto a glass slide. The oligonucleo-tide probes were hybridized with fluorescence-labeled complementary HBV DNA target molecules amplified from 40 samples that were confirmed to have single muta-tions by sequencing. This study shows that the results of the HBV drugresistance oligonucleotide chip were identical to those of sequencing and reverse hybridizati-on. The HBV drug-resistance oligonucleotide chip is very simple to use and provid-es an alternative method for the detection of HBV drug-resistance mutants. |
| Keyword | Hepatitis B virus, HBV, Lamivudine, Drug resistance, Microarray, Oligonucleotide DNA chip |
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