| Info. | Vol.11 - No.1 (2017.03.20) |
|---|---|
| Title | Rapid Visual Identification of Bovine Meat by Loop Mediated Isothermal Amplification Combined with Immunochromatographic Strip |
| Authors | Yong-Jin Li1,*, & Jin-Yu Fan2 |
| Institutions | 1Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Chinese Academy of Fishery Sciences, Huzhou University, 313000, China 2College of Life Technology, Xinxiang Medical University, Xinxiang, 453003, China *Correspondence and requests for materials should be addressed to Y.J. Li ( liyongjin@zjhu.edu.cn) |
| Abstract | Considering the factor of culture, religion, health and commerce, the developmentof a rapid, convenient and sensitive method is important for the detection of the presence of meat species in raw or processed foods. In this study, we employed loop mediated isothermal amplification (LAMP) for species-specific target gene amplification, and the amplicons were analyzed on the immunochromatographic strip (ICS) for the rapid visual identification of meat species. Bovine meat was selected as an example to confirm the performance of such assay. The LAMP based ICS method show good specificity, repeatability and has a limit of detection of 0.1% for beef in meat mixture. The whole detection process could be completed within 50 min. Our method is more convenient and rapid compared to classical LAMP. Moreover, it could be easily adapted to identify other types of meats, and it may be useful for food administration laboratories to carry out meat species identification in raw and processed foods. |
| Keyword | LAMP, Immunochromatographic strip, Species identification, PCR, Colorimetric analysis |
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