Info.
|
Vol.11 - No.2 (2017.06.20) |
Title
|
Affinity Characteristic of Terminal Sequence in Vancomycin Resistant Enterococcus (VRE) Membrane Peptides on Nanobiosensor Chip Using Localized Surface Plasmon Resonance |
Authors
|
Kyungmin Lee1, Yo-Han Kim1, Hunsang Jung1 & Hyun Ho Lee1,* |
Institutions
|
1Department of Chemical Engineering, Myongji University, Yongin 17058, Republic of Korea
*Correspondence and requests for materials should be addressed to H.H. Lee (hyunho@mju.ac.kr) |
Abstract
|
In this study, specific binding affinity between vancomycin (VAN) conjugated 40-50 nm gold nanoparticles (Au NPs) implemented on a sensor chip and pentapeptide mimicking pathogenic VAN resistant Enterococcus (VRE) wall membrane was employed as nanosensor format based on localized surface plasmon resonance (LSPR). Two pentapeptides, which were also anchored to 5 nm Au NPs, having two different terminal sequences of d-Ala-d-Ala and d-Ala-d-Lac respectively were compared in specific binding affinity toward the VAN conjugated nanosensor. Binding affinity difference identified by the LSPR characteristic in the nanosensor was shown in absorbance intensity and absorption wavelength shift between the two different terminal pentapeptides. The absorbance intensity clearly indicated that the pentapeptide having terminal d-Ala-d-Lac showed less affinity toward VAN conjugated nanosensor chip than that having terminal d-Alad-Ala. It indicates that the VRE itself has relatively weak binding strength towards the VAN, which may be used as a probe molecule for the VRE. Therefore, in order to design a VRE sensor using the VAN affinity, an indirect LSPR method can be devised instead of direct LSPR method. |
Keyword
|
Nanobiosensor, Au NPs, LSPR, Vancomycin resistant Enterococcus, Antibiotic probe |
PDF File
|
# 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다.
|