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Info. Vol.11 - No.2 (2017.06.20)
Title Application of Paper EWOD (Electrowetting-on-Dielectrics) Chip: Protein Tryptic Digestion and its Detection Using MALDI-TOF Mass Spectrometry
Authors Inae Jang1,??/sup>, Hyojin Ko1,??/sup>, Gwangro You1, Hyunji Lee1, Seunghwi Paek1, Heedo Chae1, Jae Hwan Lee2, Sunkyung Choi2, Oh-Sun Kwon1, Kwanwoo Shin1,* & Han Bin Oh1,*
Institutions 1Deparment of Chemistry, Sogang University, Seoul 04107, Republic of Korea
2CBR Defense Directorate, Agency for Defense Development (ADD), Yuseong, Daejeon 34186, Republic of Korea
??/sup>These authors contributed equally to this work.
*Correspondence and requests for materials should be addressed to H.B. Oh (hanbinoh@sogang.ac.kr) and K. Shin (kwshin@sogang.ac.kr)
Abstract A paper-based open EWOD chip was used for protein tryptic digestion, and the resulting peptides were analyzed using MALDI-TOF mass spectrometry to identify the proteins. Although on-chip protein digestion was previously demonstrated on a glass-based EWOD platform, this is the first report to show that a paper-based EWOD can also be used for protein digestion and further analysis by mass spectrometry. A number of protein digestion processes, i.e., disulfide bond reduction, alkylation, buffering, and tryptic digestion, can be successfully conducted on the paper-based EWOD chip. Furthermore, a ?쏽??shaped junction design was shown to be effective in successfully manipulating a droplet for protein digestion. A paper-based EWOD platform offers a cheap and versatile avenue for biological applications.
Keyword EWOD, Protein digestion, MALDI-TOF, Paper chip, Trypsin
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