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(사)한국바이오칩학회 The Korean BioChip Society

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Info. Vol.12 - No.4 (2018.12.20)
Title Development of Colorimetric Paper Sensor for Pesticide Detection Using Competitive-inhibiting Reaction
Authors Hyeok Jung Kim1, Yeji Kim1, Su Jung Park1, Chanho Kwon2 & Hyeran Noh1,3,*
Institutions 1Department of Optometry, Seoul National University of Science and Technology (Seoultech), 232 Gongneung-ro, Nowon-gu, Seoul 01811, Korea
2Research Institute, Biomax Co., Ltd., Seoul Technopark, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Korea
3Convergence Institute of Biomaterials and Bioengineering, Seoul National University of Science and Technology (Seoultech), 232 Gongneung-ro, Nowon-gu, Seoul 01811, Korea
*Correspondence and requests for materials should be addressed to H. Noh (hrnoh@seoultech.ac.kr)
Abstract Contamination by pesticides is an everincreasing problem associated with fields of environmental management and healthcare. Accordingly, appropriate treatments are in demand. Pesticide detection methods have been researched extensively, aimed at making the detection convenient, fast, cost-effective, and easy to use. Among the various detecting strategies, paper-based assay is potent for real-time pesticide sensing due to its unique advantages including disposability, light weight, and low cost. In this study, a paper-based sensor for chlorpyrifos, an organophosphate pesticide, has been developed by layering three sheets of patterned plates. In colorimetric quantification of pesticides, the blue color produced by the interaction between acetylcholinesterase and indoxyl acetate is inhibited by the pesticide molecules present in the sample solutions. With the optimized paper-based sensor, the pesticide is sensitively detected (limit of detection=8.60 ppm) within 5 min. Furthermore, the shelf life of the device is enhanced to 14 days after from the fabrication, by treating trehalose solution onto the deposited reagents. We expect the paper-based device to be utilized as a firstscreening analytic device for water quality monitoring and food analysis.
Keyword Pesticide, Paper microfluidic, Colorimetric sensing, Three-dimensional paper device
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