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Info. Vol.13 - No.3 (2019.09.20)
Title Automated Platform for Rapid and Reproducible Sample Preparations in Point-of-Care(POC) Molecular Diagnostics
Authors Dongkyu Lee1, Ohwon Kwon1,* , Kang-Ho Lee1, Jongsu Yun1, Changwon kim1, Joonho Seo1, Jeawan Koo2 & Gyu Man Kim2
Institutions 1Department of Medical Devices, Korea Institute of Machinery and Materials (KIMM), Daegu, 42994, Republic of Korea
2School of Mechanical Engineering, Kyunpook National Univiersity, Daegu, 41566, Republic of Korea
*Correspondence and requests for materials should be addressed to O. Kwon (owkwon@kimm.re.kr)
Abstract Fully automated system for sample preparation in molecular diagnostics was developed. The small cartridge with multiple chambers and a rotating valve was designed for automated processes such as extraction, separation and purifications during the DNA sample preparation. The automated processes such as injection, mixing, capturing and valve on/off motions were controlled by an affordable, motorized control system. We demonstrated the automated DNA extraction of pathogenic bacteria using this system. The deviations in the purity and real-time polymerase chain reaction (PCR) detection of the automated DNA extraction samples were clearly decreased. The device provided rapid, reliable, and reproducible sample preparation in comparison with manual DNA extraction. This automated platform can be utilized in emergent point-of-care testing of infectious disease and foodborne bacteria.
Keyword Molecular diagnostics, Sample preparation, Nucleic acids, Point-of-care testing, Automated platform
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