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Info. Vol.13 - No.4 (2019.12.20)
Title Electrochemical Immunoassay Based on Indium Tin Oxide Activity Toward a Alkaline Phosphatase
Authors Sunga Song1, Young Joo Kim1, Ik-Soo Shin2,* , Won-Hyo Kim1, Kook-Nyung Lee1,* & Woo Kyeong Seong1
Institutions 1Human IT Convergence Research Center, Korea Electronics Technology Institute, 25, Saenari-ro, Bundang-gu, Seongnam-si, Gyeonggi 13509, Republic of Korea
2Department of Chemistry and ICMC Conversions Technology, Soongsil University, Seoul 06978, Republic of Korea
*Correspondence and requests for materials should be addressed to I.-S. Shin (extant@ssu.ac.kr) and K.N. Lee (plummy@keti.re.kr)
Abstract In vitro diagnostic technologies, used to evaluate disease status at the point-of-care (POC), are of urgent importance to improve diagnostic capabilities in bladder cancer. However, currently available approaches have a limitation because of their lack of detection for early-staged cancer. Herein, we developed an electrochemical immunosensor for the POC diagnosis of bladder cancer in which the enzyme alkaline phosphatase (ALP) and its metabolite, ascorbic acid 2-phosphate (AAP), are employed as the electrochemical label and redox signaling unit. The indium tin oxide (ITO) electrode exhibits no redox activity toward the AAP substrate, whereas its metabolic add of ascorbic acid (AA) produces strong oxidation current under the voltammetric measurement. The ITO electrode and the ALP enzyme are revealed as an excellent combination for the development of the electrochemical immunosensors in both direct- and sandwich-type immunoassay approach. Determination of apolipoprotein-A4 and metallopeptidase- 9, two representative bladder cancer markers, were used to evaluate our approach, and excellent quantification with standard deviation 10 % was achieved. Overall, our results showed that our method offers an opportunity for the development of a variety of new forms of portable POC diagnostic devices with low cost and excellent performance.
Keyword ITO sensing electrode, The enzyme ALP, sandwich immunoassay, Enzyme labeling
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