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Info. Vol.13 - No.4 (2019.12.20)
Title High-Frequency Interdigitated Array Electrode-Based Capacitive Biosensor for Protein Detection
Authors Tuan Vu Quoc1,2, Viet Nguyen Ngoc2,3, Tung Thanh Bui3, Chun-Ping Jen2 & Trinh Chu Duc3,*
Institutions 1Institute of Applied Physics and Scientific Instrument, Vietnam Academy of Science and Technology, Hanoi, Vietnam
2Department of Mechanical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, R.O.C.
3University of Engineering and Technology, Vietnam National University, Hanoi, Vietnam
*Correspondence and requests for materials should be addressed to Trinh Chu Duc (trinhcd@vnu.edu.vn)
Abstract This paper reports a study on developing of a protein detection biochip based on interdigitated array electrodes (IDAEs) capacitive immunosensor. The protein after being preconcentrated in a detection region will be selectively captured and detected by the capacitive immunosensor. Using electrical impedance spectroscopy operated at high-frequency in the range of 100 kHz–1 MHz, the capacitance of the gold electrode is determined and the antibody surface modification steps can be also monitored. The experiment results show the capacitance changes in accordance with the adding biochemical layer on gold electrodes for each step of the antibody surface modification. In particular, the total impedance operated at 1 MHz frequency has been seen to change from 2.1 kΩ of bare chip (before antibody surface modification) to 8 kΩ after antibody surface modification process while the serial capacitance is recorded to reduce steadily from 450 pF to 55 pF. Also, the efficiency of protein chip was investigated by implementing the measurement of 10 µM BSA with and without preconcentration process. The measurement results have shown the sensitivity increasing significantly after the protein is preconcentrated in this chip. The results demonstrate high efficiency of protein detection can be achieved by operating high frequency capacitive measurement on IDAEs capacitive immunosensor.
Keyword Capacitive immunosensor, Interdigitated array electrodes, Protein preconcentration, Protein detection, Impedance spectroscopy
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