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Info. Vol.14 - No.3 (2020.09.20)
Title An On-chip Chemiluminescent Immunoassay for Bacterial Detection using in Situ-synthesized Cadmium Sulfide Nanowires with Passivation Layers
Authors Hong-Rae Kim1, Ji-Hong Bong1, Jaeyong Jung1, Jeong Soo Sung1, Min-Jung Kang2, Jae-Gwan Park2 & Jae-Chul Pyun 1,*
Institutions 1Department of Materials Sciences and Engineering, Yonsei University, 50 Yonsei-Ro, Seo-dae-mun-gu, Seoul 120-749, Korea
2Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Korea
*Correspondence and requests for materials should be addressed to J.C. Pyun (jcpyun@yonsei.ac.kr)
Abstract The passivation layers of an in situ-synthesized cadmium sulfide (CdS) nanowire (NW) photosensor were prepared for two reasons: (1) to improve the physical stability of the NW on an interdigitated electrode and (2) to enhance the immobilization efficiency of proteins for the on-chip immunoassay. The passivation layer was estimated to have suitable optical properties, and the photoresponse of photosensor was increased after the formation of passivation layer. The immobilization efficiency of a parylene-H film through covalent bonding was compared with the immobilization of Z-domains through physical adsorption. Finally, on-chip chemiluminescent immunoassay of bacteria was carried out by immobilizing antibodies against Escherichia coli through Z-domains for the orientation control of antibodies. The limit of detection was determined to be less than 104 E. coli/mL (n=3), and the sensitivity of bacterial detection was estimated to be 0.339 pA/E. coli/mL (n=3) with a linearity factor (R2) of 0.999. These results showed that the on-chip chemiluminescent immunoassay for bacterial detection could be performed using passivation layer coated CdS NW photosensor.
Keyword CdS nanowire, Photosensor, Surface passivation, On-chip immunoassay
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