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Info. Vol.2 - No.4 (2008.12.20)
Title Stimuli-Responsive Array of Microbeads Conjugated with Elastin-like Polypeptide
Authors Jonghwan Lee1, Jaeyeon Jung1, Kyunga Na1, Myeongjin Kim1, Subeom Park1, Pilwoo Heo2 & Jinho Hyun1
Institutions 1Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Korea
2Energy Plant Team, Korea Institute of Machinery & Materials, Daejeon 305-343, Korea
Correspondence and requests for materials should be addressed to J. Hyun (jhyun@snu.ac.kr)
Abstract It describes a platform of microarray for immunoassay using polystyrene microbeads (關beads) conjugated with elastin-like polypeptides (ELP) as a responsive molecule to external stimuli. Prior to the highly resolved localization of 關beads for an array, photoresist micropatterns were fabricated onto the spun-cast adhesive thin layer. A UV-curable adhesive was applied to enhance the adhesion of 關beads to the substrate by controlling UV irradiation time. Spatial placement of 關beads on the photoresist micropatterns was easily performed with a flow of colloidal suspension through a device. The 關bead array conjugated with ELP molecules was used for the immunoassay of prostate specific antigen (PSA), a cancer marker. The capture of PSA from a protein mixture was successful with high sensitivity and the release of PSA from the array was complete and reversible.
Keyword Microarray, Microbead, ELP, Immunoassay, PSA
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