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Info. Vol.2 - No.4 (2008.12.20)
Title Carboxylate-modified Latex Bead Surface for Analysis of Blood Proteins
Authors Jae-Wan Jung1, Se-Hui Jung1, Yong Hoon Kim2 & Kwon-Soo Ha1
Institutions 1Department of Molecular and Cellular Biochemistry and Institute for Vascular System Research Center
2Department of Internal Medicine, Kangwon National University School of Medicine, Chuncheon, Kangwon-Do 200-701, Korea
Correspondence and requests for materials should be addressed to K.S. Ha (ksha@kangwon.ac.kr)
Abstract We developed a new carboxylate-modified latex bead surface for determining the quantitative level of blood proteins. In this study, antibody arrays are fabricated by immobilizing a target protein-specific antibody on a carboxylate-modified latex bead surface of well-type arrays. C-reactive protein (CRP), carcinoembryonic antigen, and prostate-specific antigen were conjugated with fluorophores for use as internal standards for the analysis of blood proteins. We found that the carboxylate-modified latex bead surface had a higher binding affinity for Alexa 546-CRP than dextran, aldehyde, and amine surfaces. The carboxylate-modified latex bead surface was found to be specific and reproducible. In addition, the antibody arrays were successfully applied to the analysis of CRP, carcinoembryonic antigen, and prostatespecific antigen in normal human serum (r2=0.997, 0.988 and 0.999, respectively). Thus, an antibody array based on a carboxylate-modified latex bead surface has a strong potential for a rapid serodiagnosis of human diseases.
Keyword Antibody array, Carboxylate-modified latex bead, C-reactive protein, Carcinoembryonic antigen, Prostatespecific antigen
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