| Info. | Vol.3 - No.1 (2009.03.20) |
|---|---|
| Title | Genome-wide Inspection of Chromosomal Aberrations in Microglia BV-2 Cells by Array-based Comparative Genomic Hybridization |
| Authors | Jin Hwan Do1,2, Hyun Myung Ko2, Kyoungho Suk3, Eun Jung Park4 & Dong-Kug Choi2 |
| Institutions | 1Human Genome Center, Institute of Medical Science,
University of Tokyo, 4-6-1 Shirokanedai, Minatoku,
Tokyo 108-8639, Japan 2Bio-Food and Drug Research Center, Department of Biotechnology, Konkuk University, Chungju 380-701 Korea 3Department of Pharmacology, Kyungpook National University School of Medicine, Daegu 700-422, Korea 4Immune and Cell therapy Branch, National Cancer Center, Goyang 410-769, Korea Correspondence and requests for materials should be addressed to D.K. Choi (choidk@kku.ac.kr) |
| Abstract | A growing body of evidence indicates that microglia, resident innate immune cells in the brain, play a role in host defense and tissue repair, and function as active contributors to neuron damage in neurodegenerative disease. BV-2 microglial cells immortalized by a v-raf/v-myc recombinant retrovirus (J2) have been widely used as a microglial cell model, but there are no reports about the chromosomal characteristics of this cell line such as a gain or loss in DNA copy number. In this report we conducted a genome-wide determination of chromosomal aberrations in BV-2 microglial cells using a high-throughput, oligonucleotide array-based comparative genomic hybridization (oaCGH) technique. A segmentation method was used to divide each chromosome into segments whose probe sequences share the same relative DNA copy number on average. The genomic location of each segment was determined using the mouse genome database (UCSC mm8, NCBI Build 36). Chromosome 4 was found to have the largest gain which located in the region of chr4 : 3377972-111570775, and chromosome 3 had the largest loss segment missing from the region of chr3 : 3445973-86952997. Segments possessing more DNA copies than normal by one copy (average of log |
| Keyword | Chromosomal aberration, Microglia, BV-2, Oligonucleotide array-CGH, Segmentation |
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