| Info. | Vol.1 - No.1 (2007.03.20) |
|---|---|
| Title | Self-assembled Monolayer Fabrication of Cysteine-modified Ferredoxin |
| Authors | Jeong-Woo Choi1,2, Young Jun Kim2, Byung-Keun Oh1,2 & Moonil Kim3 |
| Institutions | 1Department of Chemical & Biomolecular Engineering, Sogang University, 1 Shinsoo-Dong, Mapo-Gu, Seoul 121-742, Korea 2Interdisciplinary Program of Integrated Biotechnology, Sogang University, 1 Shinsoo-Dong, Mapo-Gu, Seoul 121-742, Korea 3Korea Research Institute of Bioscience and Biotechnology, 52 U-Eun Dong, Yu-Sung Gu, Daejun 305-806, Korea Correspondence and requests for materials should be addressed to J.-W. Choi (jwchoi@sogang.ac.kr) |
| Abstract | Rhodobacter sphaeroides ferredoxin is a metalloprotein with ferric ion in its active site. Ferredoxin has redox property and it can transfer the electron. These molecules can be applied to the bioelectronics by fabricating them as a self-assembled bio-film. The significant key of film fabrication is the immobilization method of bio-molecule. In our previous works, it has been reported that metalloprotein film can be fabricated by using chemical linker material that have thiol-group to assemble it on gold substrate. However, the chemical linker can interfere with electron transfer because it is acted as an insulator of the system. So, we used recombinant protein with cysteine functional residue at the end of the protein which can be directly immobilized on the gold (Au) surface. It could be confirmed the immobilization of the protein and surface morphology of thin film by surface plasmon resonance (SPR) and scanning tunneling microscope (STM). These results show that cysteine-modified ferredoxin can be used for making high quality protein film, and applied to the fabrication of nano-scale bioelectronics. |
| Keyword | R. sphaeroide ferredoxin, Recombinat protein, Self assembly, Metalloprotein, Surface plasmon resonance, Scanning probe microscope, Bioelectronics |
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