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Info. Vol.3 - No.2 (2009.06.20)
Title Genetic Analysis of Seed-specific Gene Expression for Pigmentation in Colored Rice
Authors ChangKug Kim1, Shoshi Kikuchi2, Kouji Satoh2, JinA Kim3, DongHern Kim4, YongHwan Kim1,SungHan Park1, JeomHo Lee5 & UngHan Yoon1
Institutions 1Genomics Division, 3Functional Bio-Material Division and 4Bio-crop Development Division, National Academy of Agricultural Science (NAAS), RDA, Suwon 441-707, Korea
2Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki 305-8602, Japan
5On-Farm Research Division, Rural Development Administration (RDA), Korea
Correspondence and requests for materials should be addressed to U.H. Yoon (uhyoon@rda.go.kr)
Abstract Anthocyanin is a major class of flavonoids that tissues produce in response to environmental signals. The pigmentation of colored rice was analyzed by oligo microarray based on two factors, cultivar and developmental stage. As many as 250 and 350 genes were identified to be significantly up- and down-regulated. Using hypergeometric analysis for transcription factor function, the Myb and GT families, PBP, PBF, RAV, and STF factors were identified have potential anthocyanin-specific functions. We also obtained seventeen unknown genes which display novel functions. Among the genes within the GT-1 and Myb1 groups, three unknown genes were found to be upregulated significantly in both combinations of cultivar and developmental stage. These results showed functional diversity of TF families and that the biological functions of the particular TFs may be activated in a pigmentation pathway in rice.
Keyword Pigmentation, Colored rice, Microarray, Anthocyanin, Transcription factor
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