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(사)한국바이오칩학회 The Korean BioChip Society



BT+IT+NTThe Korean BioChip Society

BioChip Journal

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Info. Vol.3 - No.2 (2009.06.20)
Title Charge Storage in Redox-active Azurin Monolayer on 11-MUA Modified Gold Surface
Authors Jeong-Woo Choi1,2, Jin Seok Kim1, Sang-Uk Kim2 & Junhong Min3
Institutions 1Department of Chemical & Biomolecular Engineering, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742, Korea
2Interdisciplinary Program of Integrated Biotechnology, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742, Korea
3College of Bionanotechnology, Kyungwon University, Bokjeong-dong, Sujeong-gu, Seongnam 461-701, Korea
Correspondence and requests for materials should be addressed to J.-W. Choi (jwchoi@sogang.ac.kr)
Abstract The charge storage in the protein-based biomemory consisting of P. aeruginosa azurin was investigated. Azurin was used as an electron donor or acceptor. To immobilize the azurin on the Au surface, 11-mercaptoundecanoic acid was used as a linker material. The azurin-immobilized biofilm on Au surface was optimized by surface plasmon resonance spectroscopy and the surface morphology of protein- based biofilm on Au surface was investigated by atomic force microscope. The memory device characteristics, including the ?쐗rite?? ?쐒ead??fuctions of the selfassembled azurin biofilm, were well demonstrated with two distinct electrical states of protein-based biofilms by cyclic voltammetry. From the results, it could be concluded that the azurin biofilm could be used for the construction of nanobiochip with memory function.
Keyword Charge storage, Cyclic voltammetry, Surface plasmon resonance, Atomic force microscope, Open circuit potential amperometry, Nanobiochip
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