| Info. | Vol.3 - No.2 (2009.06.20) |
|---|---|
| Title | Zinc Ion-Mediated Concentration of Glycated Hemoglobin for Electrochemical Biosensing |
| Authors | Seo Young Son1 & Hyun C. Yoon1 |
| Institutions | 1Department of Molecular Science & Technology, Ajou University, Suwon 443-749, Korea Correspondence and requests for materials should be addressed to H.C. Yoon (hcyoon@ajou.ac.kr) |
| Abstract | We developed a biosensing strategy for glycated proteins in red blood cells. Glycated hemoglobin (HbA1C) is recognized as an important target molecule to improve the control and longterm treatment of diabetes mellitus. We employed ferrocene boronic acid (FcBA) as a signaling molecule for electrochemical biosensing, since it possesses both signaling capability and biospecificity toward the target glycated proteins. FcBA was reacted with samples containing HbA1C, and the resulting conjugate was purified and highly concentrated through treatment with zinc (Zn) ions. Zn binds to hemoglobin with an association constant of 1.3횞107 M-1. By employing Zn-mediated concentration/separation, we were able to purify HbA1C easily and rapidly. After the solution-phase purification and concentration step, electrochemical signaling was performed for HbA1C. Using cyclic voltammetry, the dynamic detection range obtained for HbA1C was around 3-15% (HbA1C per total hemoglobin), which covered the clinically important range. This technology could be implemented in a diagnostic biosensor and would be applicable to other glycoproteins that need to be analyzed. |
| Keyword | Glycated proteins, HbA1C, Hemoglobin, Zinc ion, Electrochemical biosensor |
| PDF File |
|
