| Info. | Vol.3 - No.2 (2009.06.20) |
|---|---|
| Title | ProteoChip-Based Immunoassay Method for Quantitative Determination of Serum Tumor Markers |
| Authors | Hye Jee Jang1, Yong Wan Cho1, Eun Kyoung Lee1, Yoonsuk Lee1, Il Jun Park1, Young Nam Park2, Chang Woo Lee2, In-Cheol Kang3 & Moon Hi Han1 |
| Institutions | 1Protein Chip Research Center, Proteogen, Inc., High Tech Venture Town, Chuncheon, Korea 2Central Research Center, YongDong Diagnostics, Yongin, Korea 3BioChip Research Center and Department of Biological Sciences, Hoseo University, Asan, Korea Correspondence and requests for materials should be addressed to M.H. Han (mhhan@proteogen.co.kr) |
| Abstract | Conventional biomarker assays are generally performed by an immunoassay system with several different detection methods. In this study, we have carried out validation experiments to verify performance of a novel chip-based immunoassay method for quantitative determination of tumor marker proteins by using ?쏻ell-on-a-Chip??(Well-Chip). The tumor markers, AFP and CEA, spiked in human serum matrix were analyzed by the fluorescence sandwich immunoassay method adopted for Well-Chip with arrayed mini-wells (1.5 mm diameter). Statistical analyses were carried out to validate precision, accuracy, and repeatabilityof the analytical data. Thus, the assay results obtained by the Well-Chip method were compared with those by a standard ELISA method to confirm reliability of the analytical data. It was demonstrated that quantitative assays of spiked AFP and CEA in human sera and buffer solution were possible in the linearity ranges with the Well-chip. The CV values were lower than 15% and recovery rates were within a range of 짹10% in both AFP and CEA. The correlation coefficients of analytical values obtained by within-run and between-day were higher than 0.9. The assay values of the Well-Chip method were also well correlated with those of ELISA methods. The assay rformance of the Well-Chip method was validated as a novel method for quantitative assays of tumor markers and shown to be highly dependable and reliable as much as a standard ELISA method. It is evident that the Well-Chip method can be useful or multiplex assays of biomarker proteins in human serum. |
| Keyword | ProteoChip, Tumor markers, Well-on-a-chip, ELISA |
| PDF File |
|
