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(사)한국바이오칩학회 The Korean BioChip Society



BT+IT+NTThe Korean BioChip Society

BioChip Journal

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Info. Vol.4 - No.1 (2010.03.20)
Title Differential expression of cell cycle related genes in PAH-exposed human liver cells
Authors Yu Ri An1, Seung-Jun Kim1,2, Hye-Won Park2,3, Moon-Ju Oh2, Youn-Jung Kim4, Jae-Chun Ryu4 & Seung Yong Hwang1,2
Institutions 1Department of Biochemistry, Hanyang University, Sangnok-gu, nsan, Gyeonggi-do, Korea
2GenoCheck Co. Ltd., Sangnok-gu, Ansan, Gyeonggi-do, Korea
3Department of Bionanotechnology, Hanyang University, Sangnok-gu, Ansan, Gyeonggi-do, Korea
4Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology, P.O.Box 131, Cheongryang, Seoul 130-650, Korea
Correspondence and requests for materials should be addressed to S.Y. Hwang (syhwang@hanyang.ac.kr)
Abstract Polycyclic aromatic hydrocarbons (PAHs) are one of the most widespread organic pollutants, and they can cause changes in gene expression. Biochip based microarray technologies are useful for identifying the toxicity of environmental pollutants. In this study, we performed a microarray experiment to identify the effects of PAHs in human hepatoma cells using the HazChem human array V3. The cells were exposed for 48 hours to IC20 values of four PAH chemicals (benzo[a]pyrene, dibenzo[a,h]anthracene, 3-methylcholanthrene, benzo[k]fluoranthene). Thirty of 1136 genes, which were related to the cell cycle based the ontology analysis, were commonly detected when cells were exposed to the four chemicals. Further analysis of the significantly expressed genes revealed that they affected the spindle check point. These results suggest that PAHs affect cell cycle regulation
in humans.
Keyword PAHs, HazChem, Microarray, Cell-cycle, Hepatotoxicity
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