| Info. | Vol.4 - No.1 (2010.03.20) |
|---|---|
| Title | DNA microarray analysis of gene expression profiles in Caenorhabditis elegans exposed to cadmium |
| Authors | Seok Won Jeong1, Md. Mujibur Rahman1, Jeong Won Hwang1, Jung Min Kim2, Koji Arizono3 & Young Rok Seo1 |
| Institutions | 1Department of Pharmacology, Institute for Biomedical Science Institute (IBMS), School of Medicine, Kyung Hee University, Seoul 130-701, Korea 2Department of Research and Development, Genomictree Inc., Daejeon 305-510, Korea 3Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, Japan Correspondence and requests for materials should be addressed to Y.R. Seo (dream21@khu.ac.kr) |
| Abstract | The human population exposed to cadmium from air, food and water sources may exhibit abnormalities in multiple organs including the kidneys and the liver, and in the cardiovascular, immune and reproductive systems. In addition, the recognition of cadmium biomarkers which may increase cancer susceptibility are of prime mportance. The investigation of the effects of cadmium exposure and the etermination of its uptake using animal models are vital efforts that can be made towards identifying the health effects of cadmium exposure on humans. Caenorhabditis elegans (C. elegans) is a nematode commonly used as an animal model for genetic studies and a living biomonitor and which is useful for investigating basic biological processes such as mRNA expression in response to heavy metal exposure. In the present study, we investigated gene expression hanges in C. elegans in response to cadmium chloride exposure using microarrays consisting of 22,000 nematode-oligonucleotide probes expressed (짚2 fold) following a 24 hour exposure to cadmium chloride. Several categories of genes have been detected from the microarray study and GO analysis. Based on their functions, they have been mapped according to cadmium ion responsive, we identified 958 genes, many of which are novel cadmium responsive genes, that were differentially ngiogenesis and defense response to fungus. In conclusion, our investigation suggests importantecotoxociological biomarkers to be investigated in response to cadmium chloride exposure. |
| Keyword | Cadmium chloride, Caenorhabditis elegans, Microarray, Gene ontology (GO), Biomarker |
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