| Info. | Vol.1 - No.2 (2007.06.20) |
|---|---|
| Title | Detection Technology for Antibody-Antigen Interaction on ProteoChip using Quantum Dot |
| Authors | Eung-Yoon Kim1, Soo-Ik Chang2 & In-Cheol Kang1 |
| Institutions | 1Department of Biological Science, BioChip Research Center, Hoseo University, Asan 336-795 2Department of Biochemistry, Chungbuk National University, Cheongju 361-763, Republic of Korea Correspondence and requests for materials should be addressed to I.-C. Kang (ickang@hoseo.ac.kr) & S.-I. Chang (sichang@cbnu.ac.kr) |
| Abstract | Quantum Dot (CdSe-ZnS nanocrystals, QD or Qdot) nanometer-size particles have a number of potential applications in a variety of fields. Qdots have already been put into use as an alternative to organic fluorescent dyes and fluorescent proteins. In this study, the applications of Qdots were explored in protein microarray techniques, using Angiogenin (ANG) and Anti-ANG antibody on a ProteoChip. We compared the signal-to-noise ratios between the Cy5 labeling and Qdot labeling methods. The Qdot method evidenced a higher signal-to-noise ratio than was observed in the Cy5 labeling method. This indicates that Qdots can be used as an enabling detection material for the specific quantification of proteins and profiling in protein chips. |
| Keyword | Quantum dots, ProteoChip, Protein chip, Cy5 |
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