| Info. | Vol.5 - No.2 (2011.06.20) |
|---|---|
| Title | An electromagnetically-driven microfluidic platform with indirect-heating thermo-pneumatic valves |
| Authors | Bonnie T. Chia1, Xin-Ying Yang1, Ming-Yuan Cheng1, Chii-Wann Lin2 & Yao-Joe Yang1 |
| Institutions | 1Department of Mechanical Engineering, National Taiwan University,
Taipei, 10617, Taiwan, R.O.C. 2Institute of Biomedical Engineering, National Taiwan University, Taipei, 10617, Taiwan, R.O.C. Correspondence and requests for materials should be addressed to Y.-J. Yang ( yjy@ntu.edu.tw) |
| Abstract | In this work, we present a microfluidic platform which is capable of carrying out a series of laboratorial operations on a disposable chip. The platform employs coil arrays to transport biological samples attached on magnetic beads through different reaction zones in aqueous solutions. Micromachined thermopneumatic valves are adopted as the interfaces between sequential reactions. The self-contained system is composed of a disposable microfluidic reaction (MFR) chip and a fluidic driving/sensing (FDS) module. Various reaction zones with different functionalities are implemented on the MFR chip. Arrays of coils and micromachined heater/sensing chips are integrated into the FDS module. Also, novel indirect-heating thermo-pneumatic valves (IH-TPVs), which are monolithically integrated into the disposable MFR chip, are proposed and characterized. With an applied voltage of 3.2V, the IH-TPV effectively isolates two adjacent reaction zones, while its temperature elevation is sufficiently low for biological samples and reagents. By employing the proposed approach, we design and implement a miniaturized biochip system which carries out the processes of DNA extraction, purification and amplification from whole blood sample. The dimension of the fully-integrated system is 72 mm횞54 mm횞11 mm, and the system could be fully operated with a 12 V DC power supply. DNA extraction and purification from human whole blood followed by PCR amplification for the 122 bp segment is successfully performed by using the system. |
| Keyword | Biochip, Electromagnetic, Microfluidic, Thermo-pneumatic, Valve |
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