| Info. | Vol.5 - No.2 (2011.06.20) |
|---|---|
| Title | Graphene-based electrochemical biosensor for pathogenic virus detection |
| Authors | Fei Liu1, Ki Seok Choi1, Tae Jung Park2, Sang Yup Lee2 & Tae Seok Seo1 |
| Institutions | 1Integrative NanoBioMEMS Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program) and Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu,Daejeon 305-701, Korea 2Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea Correspondence and requests for materials should be addressed to T.S. Seo ( seots@kaist.ac.kr) |
| Abstract | A graphene based biosensor system is presented for performing highly sensitive pathogenic virus detection. A free-standing conductive graphene film was prepared as a novel electrochemical sensor through two steps: synthesis of a graphene oxide (GO) film from GO colloidal suspensions by using a speed vacuum concentrator and thermal annealing process at 900??with H2/Ar flow to generate a reduced GO film. The resultant graphene film shows an excellent electron transfer property on the surface in the [Fe(CN)6]3-/4- redox system and is used as a working electrode in the electrochemical biosensor. The surface of graphene is modified with pyrene derivatives, and then covalently linked with virus-specific antibodies. The target cell, rotavirus, is captured on the graphene film through antibody-antigen interaction, and the entire process was monitored by cyclic voltammetric responses. A 105 pfu/mL of input cells is detected with ca. 30.7% sensitivity, and ca. 1.3% sensitivity is measured with 103 pfu/mL of input cells, demonstrating that graphene film based electrode can be applied for electrochemical biosensor. |
| Keyword | Graphene film, Electrochemical biosensor, Pathogen detection, Detection sensitivity |
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