| Info. | Vol.5 - No.2 (2011.06.20) |
|---|---|
| Title | Compartmented microfluidic device for positioning and chemotactic migration of cells |
| Authors | Seog Woo Rhee1 |
| Institutions | 1Department of Chemistry, College of Natural Sciences, Kongju National University, 182, 314-701, Korea Correspondence and requests for materials should be addressed to S.W. Rhee ( jisanrhee@kongju.ac.kr) |
| Abstract | This paper describes a simple approach to position cells and monitor chemotactic migrations of MDA-MB 231 human breast cancer cells. The device was fabricated in poly(dimethylsiloxane) using soft lithography and micromolding techniques. The device has three compartments with volumes less than 2 關L in each channel. The middle compartment is separated by a physical barrier in which a number of small microgrooves are embedded to allow diffusion of chemicals and migration of cells. The three-compartment diffusion system generated a steady state gradient with an exponential shape across the middle channel. It gives two extreme regions for real time monitoring of cellular behaviors: control and gradient regions. To demonstrate the utility of the device for cell migration, chemotaxis of breast cancer cells was successfully observed in a soluble gradient of chemoattractant (EGF). During the chemotaxis experiment, most cells in the control region remained along the barrier and a few cells (11.4%) migrated towards the side channel while many of the cells (31.6%) in the gradient region migrated towards the side channel containing EGF. This device is expected to be applicable as an alternative method for the investigation of chemotactic migration of cells. |
| Keyword | Microfluidics, Positioning cells, Suction technique, Chemotaxis, Breast cancer cells |
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