| Info. | Vol.5 - No.2 (2011.06.20) |
|---|---|
| Title | Computational identification of Chinese cabbage anthocyaninspecific genes |
| Authors | ChangKug Kim1,??/sup>, JinA Kim2,??/sup>, Shoshi Kikuchi3, JiWeon Choi4, YongKab Kim5, HyunJu Park2, YoungJoo Seol1, DongSuk Park1, JangHo Hahn1 & YongHwan Kim1 |
| Institutions | 1Genomics Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA), Suwon 441-707, Korea 2Bio-crop Development Division, National Academy of Agricultural Science (NAAS), Suwon 441-707, Korea 3Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki 305-8602, Japan 4Vegetable Science Division, National Institute of Horticultural and Herbal Science (NIHHS), Suwon 440-706, Korea 5School of Electrical Information Communication Engineering, Wonkwang University, Iksan 570-749, Korea ??/sup>These authors contributed equally to this work. Correspondence and requests for materials should be addressed to Y.H. Kim (yongkim@korea.kr) |
| Abstract | Therefore, 3,895 candidate genes related to anthocyanin biosynthesis in Chinese cabbage were identified using a 300K Brassica rapa microarray analysis. Gene expression during six stages of leaf developmental stages were examined in FC (green leaf) and FA(red leaf) Chinese cabbage cultivars. The 317 transcription factor genes found to be associated with anthocyanin were classified into 11 functional groups. The ratio of expression levels of each transcription factor between the two cultivars was examined during the six leaf developmental stages. A total of 14 genes were found to be expressed in all developmental stages commonly. Among these genes, 10 unknown and hypothetical genes were differentially revealed to be expressed between the two cultivars at each developmental stage, as determined by microarray analysis, and were verified by RT-PCR validation. These genes most likely play regulatory roles in either anthocyanin production or metabolism during flavonoid biosynthesis. While these genes require further validation and characterization, our results illustrate the potential usefulness of this multi-layered screening method using Chinese cabbage (Brassica rapa) microarrays. |
| Keyword | Chinese cabbage, Brassica rapa, Microarray, Anthocyanin genes, Transcription factor |
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