| Info. | Vol.5 - No.4 (2011.12.20) |
|---|---|
| Title | Development of DNA chip for jellyfish verification from South Korea |
| Authors | Gunsup Lee1, So Yun Park2, Jinik Hwang1, Youn-Ho Lee3, Seung Yong Hwang4, Sukchan Lee5 & Taek-Kyun Lee |
| Institutions | 1South Sea Environment Research Department, Korea Ocean
Research and Development Institute, Geoje 656-830, Korea
2Medical Research Center of Neural Dysfunction, Gyeonsang
National University, Jinju 660-751, Korea
3Korea Ocean Research and Development Institute, Ansan,
Gyeonggi-do 425-600, Korea
4Department of Biochemistry, Hanyang University & GenoCheck Co.
Ltd., Sangnok-gu, Ansan, Gyeonggi-do 426-791, Korea
5Department of Genetic Engineering, Sungkyunkwan University,
300 Chunchun-dong, Jangan-gu, Suwon 440-746, Korea Correspondence and requests for materials should be addressed to T.-K. Lee ( tklee@kordi.re.kr) |
| Abstract | Global warming and environmental change have been responsible for an exponential increase in the number of jellyfish. The mass propagation of jellyfish has inflicted great damage to the economy worldwide. Therefore, in order to instantly determine the possible types of jellyfish, DNA barcode data analysis was conducted using molecular markers. DNA chip technology is an efficient method for species-level identification and, as such, will contribute powerfully to taxonomic and biodiversity research. In order to identify jellyfish species, the mitochondrial COI gene, which has been shown to be widely applicable in animal barcoding, was used; the selected species-specific probe was 23 bp and was printed onto silylated slides using a robotic microarrayer. Additionally, COI genes were amplified from the genomic DNA of jellyfish using the primers LCO1490 and HCO2198 targeted to COI. In this study, we amplified and analyzed species-specific COI sequences for six jellyfish species (Aequorea coerulescens, Aurelia aurita, Bolinopsis sp., Cyanea nozakii, Dactylometra quinquecirrha, Nemopilema nomurai) collected at Jangmok Bay in Geoje. Herein, we describe each of the jellyfish species detected via microarray analysis. As a result, the COI barcode sequence technique was found to be suitable for the identification of jellyfish from South Korea. |
| Keyword | Jellyfish, DNA chip, Mitochondrial COI gene, Barcode, Microarray analysis |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
