| Info. | Vol.6 - No.1 (2012.03.20) |
|---|---|
| Title | Fabrication of a hydrophobic/hydrophilic hybrid-patterned microarray chip and its application to a cancer marker immunoassay |
| Authors | Moonkwon Lee1, Ki Hyung Kim2, Jin-Goo Park3, Jung-Hwan Lee3, Hyun-Woo Lim1, Min-Yi Park4, Soo-Ik Chang4, Eun Kyu Lee5, Dong Woo Lim2 & Jaebum Choo2 |
| Institutions | 1Semione Inc., Ansan 426-901, Korea 2Department of Bionano Engineering, Hanyang University, Ansan 426-791, Korea 3Department of Materials Engineering, Hanyang University, Ansan 426-791, Korea 4Department of Biochemistry, Chungbuk National University, Cheongju 361-763, Korea 5College of BioNano Technology, Kyungwon University, Sungnam 461-701, Korea Correspondence and requests for materials should be addressed to J. Choo ( jbchoo@hanyang.ac.kr) |
| Abstract | In this work, we report on a simple process for fabricating a hydrophobic/hydrophilic hybrid-patterned microarray chip for a fast and sensitive immunoassay. Two different types of self-assembled monolayers (SAMs) were used in the fabrication of hydrophilic well patterns and hydrophobic substrates. The hydrophilic/hydrophobic hybrid SAM pattern generates a clear-cut boundary between the sample and the background. A change in the precursor molecules allows for many different types of SAMs to be employed in the fabrication process. Fluorescence image-based detection has previously been used for the quantitative immune-analysis of a specific cancer marker. Here, a titanium-coated glass substrate was utilized to suppress auto-fluorescence signals from substrate backgrounds. Angiogenin (ANG), a small polypeptide implicated in both agiogenesis and tumor growth, was used as a target cancer marker for its validation. Assay results demonstrate that the hybrid-patterned array chip yields a narrower error deviation and a lower coefficient variation than in a conventional 96-well plate ELISA. Furthermore, the sample requirement (1 關L) for the hybrid-patterned chip is about 50 times less than that required in an ELISA (at least 50 關L). The proposed hydrophobic/hydrophilic hybrid-patterned microarray chip is expected to be a highly efficient tool that can be applied to a high throughput immunoassay of a specific cancer marker. |
| Keyword | Hydrophobic/hydrophilic pattern, SAM, Microarray, Immunoassay, Angiogenin |
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