| Info. | Vol.6 - No.1 (2012.03.20) |
|---|---|
| Title | Sensitive and multiplexed analysis of aflatoxins using time-of-flight secondary ion mass spectrometry |
| Authors | Jang-Hyuk Ahn1,??/sup>, Young-Su Jeong2,??/sup>, Tae Geol Lee3, Young-Pil Kim4 & Hak-Sung Kim2 |
| Institutions | 1Food Safety Center, R&D Institute, Namyang Dairy Co., Ltd.,
Gongju 314-914, Korea 2Department of Biological Sciences, KAIST, Daejeon 305-701, Korea 3Center for Nano-Bio Convergence, Korea Research Institute of Standards and Science (KRISS), Daejeon 305-600, Korea 4Department of Life Science, Hanyang University, Seoul 133-791, Korea ??/sup>These authors contributed equally to this work. Correspondence and requests for materials should be addressed to Y.-P. Kim ( ypilkim@hanyang.ac.kr) and H.-S. Kim ( hskim76@kaist.ac.kr) |
| Abstract | A rapid and reliable analysis of toxins is prerequisite for food control and human healthcare. Here we demonstrate a simple and multiplexed assay of aflatoxins using time-of-flight secondary ion mass spectrometry (TOF-SIMS). By simply adsorbing either a single analyte or mixed ones onto a gold substrate, the corresponding secondary molecular ions([M+H]+) were clearly observed in a single mass spectrum. As a result of concentration-dependent peak intensity, quantitative and multiplexed analysis of different aflatoxin analogs from corns was accomplished with immunoaffinity column and TOF-SIMS analysis, which showed a good correlation with HPLC data. The detection sensitivity was estimated to be as low as 10 ng mL-1. This approach presented here will find a wide application to detection of low-levels of toxins in a rapid and multiplexed way. |
| Keyword | Aflatoxin, TOF-SIMS, Quantification, Multiplexed analysis |
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