| Info. | Vol.7 - No.2 (2013.06.20) |
|---|---|
| Title | Design of gold nanoparticle and DNA oligomer conjugates for enhancement or suppression of in vitro gene expression |
| Authors | Sunho Park1 |
| Institutions | 1Department of Mechanical Engineering, Dankook University, 152 Jukjeon-ro, Suji-gu, Yongin-si, Gyeonggi-do 448-701, Korea |
| Abstract | Conjugates of gold nanoparticles and DNA oligomers (AuNP-DNA) were used in in vitro enhanced green fluorescent protein (eGFP) translation reaction. DNA sequences were designed to have varying binding energies to mRNA so that free DNA strands introduced to the reaction suppress gene expression in general. However, the amount of protein produced either increased or decreased when AuNP-DNA was used instead, depending on the concentration of AuNPDNA, coverage and the sequence of DNA strands. This result shows that AuNP-DNA?셲 specific or nonspecific adsorption to ribosomes, mRNA, and other bio-molecules can be tuned by an appropriate choice of DNA sequence, therefore AuNP-DNA can work as a biological entity that is either a gene silencing agent or a gene expression enhancer. |
| Keyword | Gold nanoparticle, DNA, Translation, Nonspecific adsorption, Ribosome, Binding energy, eGFP, Gene expression |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
