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(사)한국바이오칩학회 The Korean BioChip Society



BT+IT+NTThe Korean BioChip Society

BioChip Journal

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Info. Vol.7 - No.2 (2013.06.20)
Title Development of a DNA chip to identify the place of origin of hairtail species
Authors Jung Youn Park1,??/sup>, Ji-Hoon Kim2,??/sup>, Eun-Mi Kim1, Jung-Ha Kang1, Hyun-Suk Kang1, Cheul Min An1, Won Sun Lee2 & Seung Yong Hwang2
Institutions 1Biotechnology Research Division, National Fisheries Research and Development Institute, Gijang-gun, Busan 619-705, Korea
2Department of Biochemistry, Hanyang University & GenoCheck Co., Ltd., Sangrok-gu, Ansan, Gyeonggi-do 426-791, Korea
Abstract Hairtails of the family Trichiuridae are widely distributed in the West Sea, South Sea, and Jeju Island in Korea and form large populations on the continental shelf of the western North Pacific. These fish species are imported from China and several other
countries because of the high demand in Korea. However, imported hairtail are difficult to distinguish from domestic hairtail. Thus, we developed a DNA chip that distinguishes three hairtail species from eight countries for quick and simple species identification. Species-specific oligonucleotide probes were designed by sequence analysis of the mitochondrial cytochrome c oxidase subunit I. In this study, we used speciesspecific probes and a DNA chip system to successfully and rapidly identify three different hairtail species from eight different geographical locations.
Keyword Hairtail, DNA chip, Identification of geographical origin, Cytochrome c oxidase subunit I, Marine product
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