| Info. | Vol.7 - No.2 (2013.06.20) |
|---|---|
| Title | Optimization of a FACS based-immunoassay using E. coli autodisplaying Z-domains |
| Authors | Min Park1, Ji-Hong Bong1, Gu Yoo1, Joachim Jose2, Min-Jung Kang3 & Jae-Chul Pyun1,* |
| Institutions | 1Department of Materials Science and Engineering, Yonsei University
50 Yonsei-Ro, Seodaemun-Gu, Seoul 120-742, Korea 2Institute of Pharmaceutical and Medical Chemistry, University of Muenster Muenster, Germany |
| Abstract | We developed a fluorescence-activated cell sorter (FACS)-based immunoassay using Escherichia coli with autodisplayed Z-domains as a solid support for detection antibodies as well as a method to optimize the signal-to-noise ratio of fluorescence signals from E. coli. To improve the sensitivity of FACS measurements by eliminating artifactual signals from unrelated small particles in the samplemixture, E. coli with autodisplayed Z-domains was modified to express enhanced green fluorescence protein (eGFP) in the cytosol. By selectively gating the measurement channel for eGFP in the cytosol, we were able to discriminate between green fluorescent E. coli and artifact particles. We confirmed the efficacy of this optimization method for FACS-based immunoassay using several model reactions. Finally, as proof of concept, we used the FACS-based immunoassay and our optimization method for the medical diagnosis of human hepatitis B virus surface antigen. |
| Keyword | Immunoassay, FACS, Z-domain, Medical diagnosis, Hepatitis B virus surface antigen |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
