| Info. | Vol.7 - No.4 (2013.12.20) |
|---|---|
| Title | Laser-induced heating for cell release and cellular DNA denaturation in a microfluidics |
| Authors | Min-Sheng Hung1 & Yi-Tsung Huang1 |
| Institutions | 1Department of Biomechatronic Engineering, National Chiayi University, Chiayi 60004, Taiwan |
| Abstract | In this study, a microfluidics is integrated with a laser to develop a microfluidic system for singlecell capture and cellular DNA denaturation. The results show that the cells encountered low hydrodynamic stress at low velocities and, thus, could be captured by a cell capture structure. Laser heating of the solution at low velocity produced bubbles that pushed the cells away from the capturing areas. The released cells were subsequently lysed, and cellular DNA was extended and immobilized on the surface of pillars before undergoing laser heating to induce partial denaturation. |
| Keyword | Cell capture-release, Laser heating, DNA denaturation, Microfluidics |
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