| Info. | Vol.8 - No.4 (2014.12.20) |
|---|---|
| Title | Polydimethylsiloxane Template-based Size-selective Assembly of Single Biological Cells |
| Authors | Jae-Hyoung Park1, Seung-Ki Lee1 & Sunghwan Jung2,* |
| Institutions | 1Department of Electronics and Electrical Engineering, Dankook University, Yongin 448-701, Korea 2Department of Mechanical Engineering, Dankook University, Yongin 448-701, Korea |
| Abstract | This paper presents experimental demonstrations of size-selective parallel templated assembly of single biological cells on a polydimethylsiloxane (PDMS) template. Using the ultrasonic assembly technique known as templated assembly by selective re- moval (TASR), single chlorella cells with diameters between 5 and 20 關m were individually assembled in parallel with high size-based selectivity and precise alignment into their size-matching hemispherical assembly sites on the template. Optical observation of assembled single cells on the template without cell staining was enabled by transparency of the PDMS template. No significant change in the cell morphology was observed after assembly, confirming that the conditions of ultrasound used in the assembly are compatible. The presented size-selective trapping of single biological cells on the transparent and biocompatible PDMS tem- plate represents a biological tool with potential for direct implementation in various biological applications including cell sorting and isolation for optical cell diagnostics or cell templating for artificial tissue replication. |
| Keyword | Templated assembly by selective removal, Biological cells, Polydimethylsiloxane, Ultrasonic, Transparent and biocompatible template, Optical cell diagnostics |
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