| Info. | Vol.9 - No.1 (2015.03.20) |
|---|---|
| Title | Detection of Salmonella typhimurium by Antibody/ Enzyme Conjugated Magnetic Nanoparticles |
| Authors | Saem Mun1 & Suk-Jung Choi2,* |
| Institutions | 1Amogreentech Co., Ltd. Gangneung Science Industry Park, Block GA-13-1 #216, Gangneung 210-340, Korea 2Department of Chemistry, Gangneung-Wonju National University, Gangneung 210-702, Korea |
| Abstract | A simple, highly sensitive method was developed to detect Salmonella typhimurium using 100-nm antibody- and horseradish peroxidase-conjugated magnetic nanoparticles (Ab-HRP-MNPs). The particles bound bacteria, while unbound particles were removed by filtration. The number of bacteria was determined based on the intensity of light emitted from the chemiluminescence reaction catalysed by bacterium-bound peroxidase in the filter. The light intensity was proportional to the cells in the range of 0-104 colony forming units (CFU), with a lower detection limit of 10 CFU. |
| Keyword | Salmonella typhimurium, Magnetic nanoparticle (MNP), Immunomagnetic separation, Filtration, Antibody, Horseradish peroxidase |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
