| Info. | Vol.1 - No.4 (2007.12.20) |
|---|---|
| Title | Conjugated Biomimetic Polymer Sensors Employing Fluorescence Resonance Energy Transfer |
| Authors | Nari Kim1, Gil Sun Lee1,2& Dong June Ahn1,2 |
| Institutions | 1Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea 2Center for Integrated Nano Systems, Korea University, Seoul 136-701, Korea Correspondence and requests for materials should be addressed to D.J. Ahn (ahn@korea.ac.kr) & G.S. Lee (nanoeng@korea.ac.kr) |
| Abstract | In this study, conjugated biomimetic polymer sensors composed of 10,12-Pentacosadiynoic acid (PCDA) and fluorescent dye BO558 were prepared. Polymerized PCDA vesicles show unique colorimetric and fluorescent changes by external stresses, that is, from blue to red and from non-fluorescent to red-fluorescent. However, the quantum yield of PCDA alone is very low (blue phase: 10- 4, red phase: 0.02). Such disadvantage can be overcome via the use of fluorescence resonance energy transfer (FRET) by incorporating fluorescent dye molecules into PCDA vesicles. In this article, we report that heterogeneous PCDA/BO558 vesicle systems can selectively detect the inclusion complex of 慣-cylcodextrin by using fluorescent spectroscopy. While the fluorescence intensity of BO558 was considerably decreased in polymerized blue-phase PCDA/BO558 vesi-cles due to energy transfer, the intensity was increased and recovered upon reaction with 慣-cyclodextrin (CD). On contrary, it was not changed upon exposure to 款-CD, indicating the specific detection performance of the sensor. Such fluorescent changes are considered to result from reduced energy transfer due to a conform-ational change of the conjugated PCDA polymer backbone by the inclusion compl-exation of 慣-CD. These FRET characteristics of PCDA/BO558 vesicles would apply to a wider range of biomolecular recognition events. |
| Keyword | Polydiacetylene, Fluorescence resonance energy transfer (FRET), Inclusion complex, Biomolecular recognition |
| PDF File |
|
