| Info. | Vol.1 - No.4 (2007.12.20) |
|---|---|
| Title | Improvement of DNA Chip Performance by Using a Silver-patterned Chip Substrate |
| Authors | Mikyung Kim1 & Changwoo Park2 |
| Institutions | 1Advanced Nano Products Co., Ltd, 244 Buyong Industrial Complex, Kumho-ri, Buyong-myeon, Cheongwon-kun, Chungcheongbuk-do 363-942, Korea 2Division of Applied Chemistry & Biotechnology, Hanbat National University, san 16-1 Duckmyoung-dong, Yuseong-gu, Daejeon 305-719, Korea Correspondence and requests for materials should be addressed to M. Kim (Mikyung-kim@anapro.com) |
| Abstract | We have applied the inkjet technology to the development of Ag-patterned biochip substrates in order to increase the production capability, the sensitivity, and the detection limit of biochips. Although silver has a potential to be a better substrate for biosensors and biochips than gold because of its higher chemical affinity toward biomaterials, conventional Ag films have a high reactivity and poor adhesion to substrates (especially glass), and the films can be easily destroyed during the process of biochip fabrication. However, these problems in conventional silver films were overcome by using the unique ANP Ag ink and ink jet technology, which produced a highly stable and adhesive silver film on glass substrates. By applying this inkjet technology, we were able to produce a great variety of patterns in chip substrates, increase the immobilization efficiency by more than 2 times when compared with conventional substrate technology, and increase the chip sensitivity to allow a 100 times reduction in the amount of probe needed. We also investigated the chip specificity by applying the perfect match and mismatch samples into a silver-patterned chip. These results show that a Ag-patterned chip substrate with high sensitivity and specificity can be used for the application of biochip and biosensors. |
| Keyword | Silver nanoparticles, DNA chip substrate, Inkjet printing, Chip Sensitivity |
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