| Info. | Vol.9 - No.4 (2015.12.20) |
|---|---|
| Title | Optimization of Specific Multiplex DNA Primers to Detect Variable CLU Genomic Lesions in Patients with Alzheimer?셲 Disease |
| Authors | Vo Van Giau1 & Seong Soo A. An1,* |
| Institutions | 1Department of Bionano Technology & Gachon Medical Research Institute, Gachon University, 1342 Sungnam daero, Sujung-gu, Seongnam-si, Gyeonggi-do 461-701, Korea |
| Abstract | Recently, polymorphisms in the clusterin (CLU) or Apolipoprotein J (ApoJ) gene were reported to be involved in lipid metabolism, atherogenesis, and being associated with the risk of developing Alzheimer?셲 disease (AD). The influences from genetic variation has not been examined among Koreans. To screen genes with abnormal exon expression profiles, we developed PCR primer pairs for CLU gene. The new primer set can target specific regions of previously sequenced CLU gene. Primers were designed to target eight exon amplicons with flanking regions to optimize PCR amplification. One-hundred samples from clinically diagnosed Korean AD patients were selected for testing. We identified four single nucleotide polymorphisms (SNPs) in CLU through direct sequencing of the PCR products. These included three SNPs (rs7982, rs2279590 and rs3216167) that were previously reported variants in the SNP database (dbSNP), which could be found in NCBI. Interestingly, one new (NEW1) or extremely low-frequency mutation was found in more than one individual among the late onset AD subjects. Our study suggests that CLU variants may also be an AD susceptibility factor among Koreans. Moreover, the findings of the study can be able to develop for simultaneous identify all of the genotypes of CLU mutation sites by DNA microarray PCR-based in the future. |
| Keyword | Clusterin, CLU variants, Alzheimer?셲 disease, PCR analysis, DNA primers, SNPs |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
