| Info. | Vol.10 - No.3 (2016.09.20) |
|---|---|
| Title | Tumour-like Druggable Gene Expression Pattern of CaCo2 Cells in Microfluidic Chip |
| Authors | Timur R Samatov1,2,*, Nadezhda V Senyavina3, Vladimir V Galatenko4, Eugene V Trushkin1,Svetlana A Tonevitskaya1,2, Dmitriy E Alexandrov4, Galina P Shibukhova3,Udo Schumacher5 & Alexander G Tonevitsky3,4,* |
| Institutions | 1Hemule GmbH, Rudower Chaussee 46, 12489 Berlin, Germany 2Moscow State University of Mechanical Engineering, Bolshaya Semenovskaya str 38, 107023 Moscow, Russia 3P. Herzen Moscow Oncology Research Institute, National Center of Medical Radiological Research, 3 Second Botkinsky Lane, Moscow, 125284, Russia 4Moscow State University, Leninskie Gory, 119991 Moscow, Russia 5Department of Anatomy and Experimental Morphology, University Cancer Center, University Medical Center Hamburg-Eppendorf, Martinistr. 52, Hamburg D-20246, Germany *Correspondence and requests for materials should be addressed to T.R. Samatov ( t.samatov@hemule.de) & A.G. Tonevitsky ( tonevitsky@mail.ru) |
| Abstract | The human-on-chip technology provides an efficient basis for preclinical studies and has potentially a greater predictive power for human drug response than classical 2D cell culture. Here we report the expression profile of druggable genes in the human colon cancer cells CaCo2 in static culture and within a microfluidic chip. We identified gene expression pattern under flow to be closer to the one of CaCo2 primary xenograft tumours as compared to those cells grown without circulation. The obtained results indicate that a microenvironment connected to a circulation within a chip brings the cells closer to in vivo situation. Hence the human-on-chip technology is a more powerful tool for drug development than conventional 2D cell culture. |
| Keyword | CaCo2 cell line, Microfluidic chip, Microcirculation, Microarray analysis, Druggable genes |
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