| Info. | Vol.10 - No.3 (2016.09.20) |
|---|---|
| Title | Quorum Sensing is Crucial to Escherichia coli O157:H7 Biofilm Formation under Static or Very Slow Laminar Flow Conditions |
| Authors | Jeesun Lim1, Kang-Mu Lee2, Chan Yong Park3, Han Vit Kim4, Younghoon Kim5 & Sungsu Park3,4,* |
| Institutions | 1Departiment of Chemistry and Nano Sciences (BK21 plus), Ewha
Womans University, Seoul, Republic of Korea 2Department of Microbiology, College of Medicine, Yonsei University, Seoul, Republic of Korea 3Department of Global Biomedical Engineering, Sungkyunkwan University, Suwon, Republic of Korea 4School of Mechanical Engineering, Sungkyunkwan University, Suwon, Republic of Korea 5Department of Animal Science and Institute of Milk Genomics, Chonbuk National University, Jeonju, Republic of Korea *Correspondence and requests for materials should be addressed to S. Park ( nanopark@skku.edu) |
| Abstract | Quorum sensing (QS) is defined as a cell-to-cell signaling process that collectively regulates the gene expression of bacteria via small signaling molecules called autoinducers (AIs). It was reported that QS-regulated gene expression in Pseudomonas aeruginosa failed to occur at a high Reynolds number (Re=3,000), since AI-2, a secreted interspecies signaling molecule, was washed away and so could not reach the minimum concentration required for QS. In this study, we describe the effects of flow speed on QS-stimulated biofilm formation in Escherichia coli O157:H7 inside a very thin microchannel (3 cm횞1 cm횞45 關m). In microtiter plates, the wild-type strain produced high amounts of exopolysaccharide, whereas its QS mutants ?luxS and ?lsrK, defective in AI-2 production and phosphorylation, made less exopolysaccharide. Confocal laser scanning microscopy showed that at a flow rate of 1 關L/min the wild-type strain formed rounded biofilms, whereas such biofilms formed by the QS mutants were fewer and thinner. At a flow rate of 10 關L/min, none of the tested strains formed mature biofilms. Our results suggest that QS is essential in the biofilm maturation under static or very slow laminar flow conditions where the biofilm signal can be easily accumulated and transported to the sessile cells. |
| Keyword | Escherichia coli O157:H7, Biofilm, Quorum sensing, Laminar flow, Autoinducer-2 |
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