| Info. | Vol.10 - No.4 (2016.12.20) |
|---|---|
| Title | Rolling Circle Amplification as Isothermal Gene Amplification in Molecular Diagnostics |
| Authors | Nam-In Goo1 & Dong-Eun Kim1,* |
| Institutions | 1Department of Bioscience and Biotechnology, Konkuk University, 120
Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea *Correspondence and requests for materials should be addressed to D.-E. Kim (kimde@konkuk.ac.kr) |
| Abstract | Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases. The product of RCA is long single-stranded DNA that contains tandem repeat of target sequence. Isothermal reaction amplification condition of RCA has an advantage over conventional polymerase chain reaction, because no temperature cycling devices are needed for RCA. Thus, RCA is suitable tool for point-of-care detection of target nucleic acids as well as facile detection of target genes. Combined with various detection methods, RCA could amplify and detect femtomolar scale of target nucleic acids with a specificity of one or two base discrimination. Herein, RCA technology is reviewed with an emphasis on molecular diagnosis of microRNAs, infectious pathogens, and point mutations. |
| Keyword | Rolling circle amplification, Isothermal DNA amplification, Molecular diagnostics, Micro RNA, Single Nucleotide Polymorphisms |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
