| Info. | Vol.11 - No.3 (2017.09.20) |
|---|---|
| Title | PDMS Microchannel Surface Modification with Teflon for Algal Lipid Research |
| Authors | Jae Woo Park1,??/sup>, Sangcheol Na2,4,??/sup>, Myeongwoo Kang2, Sang Jun Sim5 & Noo Li Jeon2,3,4,* |
| Institutions | 1Seoul Center, Defense Agency for Technology and Quality (DTaQ), Seoul 04505, Republic of Korea 2Division of WCU (World Class University) Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 08826, Republic of Korea 3School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 08826, Republic of Korea 4Institute of Advanced Machinery and Design (SNU-IAMD), Seoul National University, Seoul 08826, Republic of Korea 5Department of Chemical and Biological Engineering and Green School, Korea University, Seoul 02841, Republic of Korea ??/sup>These authors contributed equally to this work. *Correspondence and requests for materials should be addressed to N.L. Jeon (nljeon@gmail.com) |
| Abstract | This paper presents a simple method for modifying the polydimethylsiloxane (PDMS) microfluidic channels with Teflon for algal lipid research. When culturing and staining algae inside microfluidic devices, the small molecule dyes absorbed by the microchannel surface render it difficult for imaging and quantification. PDMS surface coated with Teflon-AF resists the absorption of hydrophobic dye molecules (i.e., BODIPY and Nile red) as confirmed using fluorescence microscopy. Here, we introduce a surface modification of PDMS microchannel using Teflon-AF using a procedure of filling and drying to directly treat the PDMS surface with perfluorinated materials. This method can be used to prevent the absorption of fluorescent probe and obtain clear fluorescence micrographs without background signal from absorbed dye molecules on PDMS microchannel. We confirmed that contact angle of Teflon-coated PDMS (116.4째) is higher than that of unmodified PDMS (106.1째) and thus more hydrophobic. Furthermore, Teflon-coated PDMS surface had ~80% of oxygen transfer rate compared to that of native PDMS and good transparency in all visible light regions. Based on these characteristics, we successfully validated the visualization and quantification of intracellular lipid droplets in microalgae C. reinhardtii using BODIPY. We believe that our new method will expand microfluidic applications on characterization of biological lipid with fluorescence probes and biochemical markers. |
| Keyword | Microfluidic device, Molecule absorption, Teflon coating, Microalgae, Chlamydomonas reinhardtii |
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