| Info. | Vol.12 - No.1 (2018.03.20) |
|---|---|
| Title | Label-free Microarray-based Binding Affinity Constant Measurement with Modified Fluidic Arrangement |
| Authors | Junwei Hu1, Ru Chen2, Chenggang Zhu2, Bilin Ge2, Xiangdong Zhu3, Lan Mi2, Jiong Ma2, Caiqin Han1, Hao Chen1,* & Yiyan Fei2,* |
| Institutions | 1Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou, 221116, China 2Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai, 200433, China 3Department of Physics, University of California, Davis, California, 95616, USA *Correspondence and requests for materials should be addressed to Y. Fei (fyy@fudan.edu.cn) and H. Chen (6019980049@jsnu.edu.cn) |
| Abstract | The oblique-incidence reflectivity difference (OI-RD) scanning microscopy has the capability of simultaneously measuring binding curves of a protein probe with tens of thousands molecular targets in a microarray and yielding reaction rate constants. However, the quality of reaction rate constants is influenced by the fluidic system. To improve the quality of reaction rate constant measurement over the entire microarray, we demonstrate a fluidic chamber that allows the fluid to flow from the bottom to the top uniformly across the microarray and thus provides more uniform and accurate measurements of reaction rate constants with simplified fluidic design. |
| Keyword | Optical biosensors, Protein microarray, Kinetics measurement, Affinity constant |
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