| Info. | Vol.12 - No.1 (2018.03.20) |
|---|---|
| Title | DAQ based Impedance Measurement System for Low Cost and Portable Electrical Cell-Substrate Impedance Sensing |
| Authors | Il-Hwan Park1, Yeonhee Hong2, Hee-Sook Jun2,3,4, Eou-Sik Cho5 & Sungbo Cho1,4,* |
| Institutions | 1Department of Biomedical Engineering, Gachon University, 191 Hambakmoe-ro, Yeonsu-gu, Incheon 21936, Republic of Korea 2College of Pharmacy and Gachon Institute of Pharmaceutical Science, Gachon University, 191 Hambakmoe-ro, Yeonsu-gu, Incheon 21936, Republic of Korea 3Lee Gil Ya Cancer and Diabetes Institute, 155 Get-Pearl-ro, Yeonsu-gu, Incheon 21999, Republic of Korea 4Gachon Advanced Institute for Health Science & Technology, Gachon University, 155 Get-Pearl-ro, Yeonsu-gu, Incheon 21999, Republic of Korea 5Department of Electronic Engineering, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-si, Gyeonggi-do 13120, Republic of Korea *Correspondence and requests for materials should be addressed to S. Cho (sbcho@gachon.ac.kr) |
| Abstract | Electric cell-substrate impedance sensing (ECIS), a technique for label-free and real-time detection of cells, is emerging as an alternative or assistive method to traditional biochemical assays for diagnostic and pharmaceutical applications. In this work, we developed a DAQ based impedance measurement system for low cost and portable ECIS applications. The alternating input signal for ECIS was generated from the digital-to-analog converter (DAC) channel of the DAQ board and applied through the current limited resistor to the cells on the electrode. The responding signal was recorded by the analog-to-digital converter (ADC) channel of the DAQ board, and its amplitude and phase were analyzed by software lock-in amplifier. The feasibility of the developed ECIS system was evaluated by impedance measurement and DAPI analysis of C2C12 cell growth on the indium tin oxide microelectrode, and by impedimetric and MTT cell viability assay of HEK293 cells exposed to H2O2. |
| Keyword | Cell viability assay, DAQ, Electric cell-substrate impedance sensing, Indium tin oxide electrode, Software lock-in amplifier |
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